As a leading enterprise in China’ s analytical and measurement industry, Skyray Instrument set up “Research Center of Applications” to provide responsive solutions to hot issues in society, especially public safety issues.
The problem of “swill-cooked oil” has existed for a long time and received a lot of attention from people, however, it hasn’t been solved yet. The recent outbreak of “swill-cooked oil” has again caused wide public concern. It is reported that consumption of low-dose aflatoxin in the swill-cooked oil over a long period of time will lead to cancer. Food safety is critical to people’s livelihood, which we regard it as an obligation of our own.
With regard to the “swill-cooked oil” event, a group of experts is organized promptly within our company to work on the analytical testing method and solutions.
You can log on our website or dial our service hotline for more information and our solutions!
Our website: www.skyray-instrument.com
Hotline: 800-9993-800 400-7102-888
The limit of AFT b1 has been clearly stipulated in relative food hygiene standards, where, corn, peanut kernel and peanut oil shall not contain b1 over 20ug/kg; rice and other edible oils shall not contain b1 over 10ug/kg; other foodstuff, beans and ferment food shall not contain more than 5ug/kg b1; food for baby shall not contain any b1.
For the test of excessive AFT b1 and combined with relative standards, high-performance liquid chromatograph-fluorescence detector is developed to conduct the test, so as to ensure the healthy edible oil.
Detailed methods for testing the limit of aflatoxin b1 in swill-cooked oil via LC310 are as follows:
P310 high-pressure delivery pump：2 setsLC310 HPLC Configurations
High-pressure static mixer（1.7ml）：1set
7725i manual injector：1 set
Post-column device：1 set
Fluorescence detector：1 set
Binary gradient kit：1 set
CT310 Chromatographic column: 1 set
LC310 Chromatographic data processing system：1 set
Chromatographic column：Kromasil C18 250*4.6mm，5um，100A or equivalent one: 1 pcs
Solvent tray：1 set
Pre-treatment equipments:
Thermostat water bath: 1set
Filter ( with vacuum pump)1000ml ： 1 set
Homogenizer（18000r/min-22000r/min）：1 set
Aflatoxin Immuno-Affinity Column: 1 pcs
Glass fiber filter: Several
Glass injector: 10ml，20ml：several
Glass tube：Diameter in 12mm; length:75mm; without fluorescence: 2 pcs
Air-pressure pump：1 set
Ultra-sonic cleaner：1 set
Laboratory reagents:
Acetonitrile: (chromatographic pure)
Methyl alcohol: (chromatographic pure)
Benzene: (chromatographic pure)
Sodium chlorine: (AR)
Water: ultrapure water or redistilled water
Disodium hydrogen phosphate: (AR)
Monopotassium phosphate: (AR)
Potassium Chloride: (AR)
Polysorbate 20: (AR)
Iodine: (AR)
Sample extraction:
Reference: GB/T18979-2003
Extract the sample through methanol alcohol-water; after the extracted solution is filtered and diluted, process the filtrate through the immunoaffinty chromatography purification of the aflatoxin antibody; remove the impurities on the immunoaffinty chromatographic column with water or polysorbate 20, elute immunoaffinty chromatographic column in methanol alcohol; test aflatoxin b1 content in eluent through post column iodine solution derivatization with High-efficiency Liquid Chromatograph, combined with fluorescence detector.
Chromatographic condition:
Mobile phase: methanol alcohol: water=45:55
Flow rate: 0.8ml/min
Post column derivatization system
Derivatives solution: 0.05% iodine solution
Derivatives solution flow rate: 0.2ml/min
Reaction tube temperature: 70℃
Reaction time: 1min
Personnel with experience in chemical analytical instruments and related majors or operation of High-frequency Liquid Chromatograph
